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Optimisation of ecotoxicological test methods
for routine use (ERNTE) |
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SP 1: "Validation of standardised test systems and coordination of the joint SP 2: "Biological test procedures for assessing the quality of soil materials - SP 2.1: "Aqueous dischargeable toxic potential" - SP 2.2: "Microbial respiration activity" - SP 2.3: "Software for automation and evaluation" SP 3: "Preparatory investigations for the development of a test kit for the solid contact test with Arthrobacter globiformis"
The German Federal Soil Protection Act (BBodSchG) names groundwater and soil, in its function as a habitat for soil organisms and plants, as major targets for protection. The Federal Soil Protection and Contaminated Sites Ordinance determined soil values for priority contaminants. However, for an assessment of contaminated soils or soil materials referencing just a few pollutants is not sufficient. In contrast, the application of ecotoxicological tests enables the integrated evaluation of all pollutants. Such an approach requires one to discriminate between the assessment of an impairment of the soil as a habitat for soil organisms and plants and the assessment of the water extractable toxic potential (soil is the protective medium for groundwater). Since the beginning of the 1990s, there have been intense efforts at both the national and international (e.g. EU, ISO) level to develop suitable and standardised test methods for appraising both soil habitat and groundwater protection. The aim of the ERNTE joint research project is to improve the assessment of soil quality by application of ecotoxicological tests under practical conditions (i.e. routine use). Part of this goal is to define limits for already established test methods with soil organisms (SP 1). Within the scope of an on-site-analysis it is important to get results quickly and with minimal effort. Thus, the duration of test methods has to be shortened and the degree of automation increased (SP 2). For the simultaneous detection of contaminants dissolved in pore water and adsorbed to the soil matrix a test using soil suspensions has to be advanced (SP 3). The results of the joint research project will be precise recommendations for the routine use of ecotoxicological test methods in addition to existing physico-chemical and chemical methods for the assessment of soil quality.
The partners of the joint research project will undertake the following tasks: SP 1: Investigation of the influence of natural soil characteristics on the results of ecotoxicological tests using the earthworm, collembolan, and plant tests as examples. SP 2: Optimisation of the umu-genotoxicity test and the Vibro fischeri luminescence inhibitory test for the determination of the aqueous dischargeable toxic potential and optimisation of respirometric investigations of soil and soil material: time reduction of both sample preparation and test performance for the on-site characterisation of contaminated land, especially in parallel to remediation processes; automation of the process, analysis, evaluation and documentation of the investigation by means of a standardised and user-friendly software. SP 3: Investigation of the influence of soil characteristics on the solid contact test and its optimisation (miniaturisation) for development of a test kit with conserved bacteria; thus biological test methods shall be used for an efficient assessment of soil or soil substrates on middle-term.
ECT Oekotoxikologie GmbH FU Berlin, FB Geowissenschaften, FU-ENVOG RWTH University Hospital Aachen, Institut of Lange GmbH & Co KG IME Fraunhofer Institut for Molecular Biology and Bio Metric Systems GmbH Hölle & Hüttner AG Informationstechnologie Dr. Fintelmann und Dr. Meyer Handels- und TU Hamburg-Harburg, AB Umweltschutztechnik
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| (c)2002 by ECT | :Sub-Projects: | :Primary Goals: | :Investigations: | :Further Information: |
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